Air Microbiology Dynamics in Operating Theatre at rest and in operational: case study at Academic Hospital Prof. Mulyanto, Mataram University

Wardoyo, Eustachius Hagni and Yudhanto, Didit and Lestari, Rina and Ajmala, Indana Eva and Sabrina, Yunita (2018) Air Microbiology Dynamics in Operating Theatre at rest and in operational: case study at Academic Hospital Prof. Mulyanto, Mataram University. Proceeding The 1st International Scientific Meeting on Clinical Microbiology and Infectious Diseases Surabaya Oct 10-14 2018, 1 (1). (In Press)

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Introduction: Hospital as reservoir of numerous microbial pathogen is assumed to contribute of HAIs incidence. One of environmental reservoir is air microbiology in high risk of transmission rooms such as operating theatre in hospital. Purpose: To describe air microbiology dynamics in operating theatre (OT) at Academic hospital Prof. Mulyanto at rest and in operational. Methods: Measurement and sampling at rest: air sampling with settle plate with blood agar plate (BAP) in 5 points, environmental swabs in 5 points, air humidity, air temperature, concrete moisture all measured in duplo. In operational=at rest, add with nasal swab samples of surgery staff, activity load, all was measured in 3 surgery procedures. Lastly air change hours (ACH) rate. In operational condition, air, surface swab and nasal microbiology with similar morphological colony on BAP preceed to culture and antibiogram in pair. At rest state=OT is not operated 8 hours or more with ventilation off. Results: Measurement in at rest and operational as follow: air microbiology 8.8 cfu/m3 and 14.9 cfu/m3, the mean of air temperature 27.8°C (27.6-28) and 28.4°C (28.2-28.8), air humidity 58%(58-58) and 56% (56-56), concrete moisture 22.45%(21.8-23.1) and 22.24%(18.67-26.03). Activity load was 23 people (18-27). Volume OT is 109,41 m3 with air circulation of 6,2 ACH. Similar morphology of colony found and confirmed by Gram stain found in 10 pairs of BAP= 8 pairs of Gram positives bacilli and 2 pairs of Gram positives cocci. Microbial identification found only one pair (3A1S1) with similar Shigella dysenteriae (Group A) isolate with comparable antibiogram which found in air microbiology and surface swab sample pair. Conclusions: Air microbiology at rest was less than 10 cfu/m3 fitted to ministry of health regulation. Contribution in increase number of air microbiology in operational compared to at rest was higher air temperature, lower air humidity and concrete moisture and surgery staff activities. Only one pair of similar isolate found, which is surface microbes contribute as air microbiology left unexplained.

Item Type: Article
Keywords (Kata Kunci): air microbiology, bioaerosols, operating theatre
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Kedokteran
Depositing User: dr.Sp.MK Eustachius Hagni Wardoyo
Date Deposited: 17 Nov 2018 01:21
Last Modified: 17 Nov 2018 01:21

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