Analysis of Genetic Mutations of Anti-malarial Resistance Genes in Nusa Tenggara Regions and Production of 19-kDa Fragment of Merozoite Surface Protein 1 as Malaria Vaccione Candidate

Muhammad ALi, .Ali and Made Sriasih, Sriasih and Sulaiman N. Depamede, Sulaiman and Tertawindu A H, Tertawindu and Ahmad Taufiq, Taufiq and Yasa Asmara, Yasa and Yunita Sabrina, Yunita (2011) Analysis of Genetic Mutations of Anti-malarial Resistance Genes in Nusa Tenggara Regions and Production of 19-kDa Fragment of Merozoite Surface Protein 1 as Malaria Vaccione Candidate. News from the National Academy of Sciences, Indonesia.

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Abstract

Malaria, sometimes called the “King of Diseases”, is a leading og mortality and morbidity globally. There were 247 million cases among 3.3 billion people at risk in 2006 from 109 countries resulting in estimated 881,000 deaths (WHO, 2008). Excessive use of antimalarial drugs resulting in drug pressure that promotes the spread of resistance to antimalarial drugs. The drug resistance is caused by point mutation at pfcrt and pfmdr1 (resistance to chloroquine) and dhfr and dhfs (resistance to sulfadoxin-pyrimethamine). The aim of this research was to observe these mutations in the genes of plasmodium falciparum-infected patients in Nusa Tenggara region. To anticipate the antimalaria treatment failure, MSP119 gene was cloned and expressed to generated blood-stage vaccine candidate of malaria. Research result showed that mutations at codon 76 in the pfcrt and 1034 in the pfmdr1 are present in all samples, indicating that potential chloroquine treatment failures may occur in all areas of Nusa Tenggara. Mutations at codon 613 of the dhfs and codon 108 in the dhfr are in very low prevalence indicating that sulfadoxin-faciparum was cloned into pET22b vector and expressed seccessfully in E. coli BL21 Star (DE3)pLysS cells. The purified MSPI19 was obtained using BD TalonTM metal affinity resin. The protein then could be used for anti-MSPI19 monoclonal antibody production and serological detection of malaria.

Item Type:	Other
Keywords (Kata Kunci):	Malaria, King of Diseases, mortality, morbidity globally
Subjects:	R Medicine > R Medicine (General)
Divisions:	Fakultas Peternakan
Depositing User:	Ali Muhamad Ali
Date Deposited:	26 Oct 2020 03:03
Last Modified:	26 Oct 2020 03:03
URI:	http://eprints.unram.ac.id/id/eprint/17919

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