Cloning and Expression of 19-kDa Fragment of Merozoite Surface Protein-1 (MSP-119) of Plasmodium Falciparum in Escherichia coli

Ali, Muhamad and Made Sriasih, Sriasih and Depemade, Sulaiman N and AH, TETRAWINDU and TAUFIQ, AHMAD and ASMARA, YASA and Sabrina, Yunita Cloning and Expression of 19-kDa Fragment of Merozoite Surface Protein-1 (MSP-119) of Plasmodium Falciparum in Escherichia coli. In: International Seminar On Economic, Culture And Environment.

Text 5. SI-04 Coning and expression of 19kDa fragment.pdf Download (1MB)

Abstract

Malaria caused by Plasmodium falciparum is a disease affecting 300 to 500 million people in tropical countries including Indonesia annually. Out of several ongoing eradication strategies against the disease, vaccine development represents an encouraging approach for improved malaria control globally. The C-terminal 19 kDa fragment of the P. falciparum merozoite surface protein 1 (MSP119), a surface protein of merozoite which plays a pivotal role in binding of merozoite to erythrocytes, has been developed as potential vaccines against erytrocytic stages of malaria. In vitro studies show that monoclonal and polyclonal antibodies specific to this protein block the entry of merozoite into erythrocytes. The aims of this study were to clone and to express the MSP119 of P. falciparum so that the effective vaccine could be produced. Moreover, the availability of the antigen will facilitate the monoclonal and polyclonal antibodies development. For these purposes, genomic DNAs of P. falciparum were isolated and were used as a template to amplify a DNA encoding the MSP119. Recombinant plasmids were constructed by insertion of the isolated PCR product into bacterial vectors of pGEMT-Easy for cloning and pET-22b for expression. In this paper, we reported that the gene encoding the MSP119 of P. falciparum was successfully amplified from P. falciparum genomic DNAs as shown by the 294 base pairs PCR product on agarose gel electrophoresis. Sequencing analysis confirmed that there are no base pair changes in the sequence of the MSP119. Preliminary result on expression of the MSP119 of P. falciparum indicated that the gene was successfully produced in E. coli.

Item Type:	Conference or Workshop Item (Speech)
Subjects:	Q Science > QR Microbiology S Agriculture > SF Animal culture
Divisions:	Fakultas Kedokteran Fakultas Peternakan
Depositing User:	. Zulkarnain Zulkarnain
Date Deposited:	06 Nov 2020 08:22
Last Modified:	06 Nov 2020 08:22
URI:	http://eprints.unram.ac.id/id/eprint/18238

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