Ali, Muhamad and N. Depamede, Sulaiman and Amin, Muhamad and Sukarne, Sukarne Molasses as an Alternative Low-cost Medium for Production of Recombinant vp28-Antigen in Escherichia coli. In: International Conference on Mathematics, Sciences and Education, University of Mataram 2015 (ICMSE 2015), 4-5 Nopember 2015, Mataram, Lombok, Indonesia.
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Abstract
Previous studies reported that VP28, which is a capsid protein of White Spot Syndrome Virus (WSSV), showed to be a potential candidate for WSSV vaccine. However, the large-scale production of the protein is hampered by the availability and expensive cost of culture media. Thus, this study was aimed at finding alternative low-cost medium for growing bacterial hosts carrying plasmid for producing VP28 proteins fused with glutathione s-transferase (GST) enzyme which then indicated as GST.VP28 fusion protein. The result showed that 7.5% molasses seemed to have better growth than commercial media (LB). Other result also indicated that quality of GST.VP28 protein expressed by host in 7.5% molasses appeared to be good. Ass indicated by its solubility. This result suggested that molasses medium can be an alternative low-cost medium for large-scale production of GST.VP28 protein.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Keywords (Kata Kunci): | white spot syndrome virus (WSSV), VP28, molasses. |
| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Fakultas Peternakan |
| Depositing User: | . Zulkarnain Zulkarnain |
| Date Deposited: | 06 Nov 2020 08:22 |
| Last Modified: | 06 Nov 2020 08:22 |
| URI: | http://eprints.unram.ac.id/id/eprint/18240 |
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