Ali, Muhamad Turnitin: Molecular Cloning of Immunoglobulin Genes Repertoire from a Variety of Animals Using a Robotic System. Mataram University Pres.
|
Text
23. SI-05 Molecular Cloning turnitin result.pdf Download (3MB) | Preview |
Abstract
Cloning and expression of immunoglobulin light chain (Lc) and heavy chain (Hc) genes from a single cell of hybridoma and immunized mouse have been previously reported (Ali et al., 2006; Sabrina et al., 2010). Since the cloning relies on a manually single cell isolation and a reverse transcription polymerase chain reaction (RT-PCR), however, the recovery ratio of coupled Lc and Hc genes obtained from the single cell was very low (11.3%). Here, a cloning of the immunoglobulin genes (Ig-genes)from a variety of animals (mouse, rat, and Guinea pig)cells was performed by the use of a robotic system. A single plasma cell of immunized animals was isolated using a fluorescent-activated cell sorting (FACS)and subsequently continued with cDNA synthesis in free-hand handling procedures. The results showed that the recovery ratio of amplified Lc and Hc genes from a variety of animals cells was increased up to 60-90%. This method is useful not only to obtained larger antibodyencoding gene from animal repertoires but also by-pass some steps which laborious, time consuming, and prone to contamination
| Item Type: | Other |
|---|---|
| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Fakultas Peternakan |
| Depositing User: | . Zulkarnain Zulkarnain |
| Date Deposited: | 11 Feb 2021 00:37 |
| Last Modified: | 11 Feb 2021 00:37 |
| URI: | http://eprints.unram.ac.id/id/eprint/20908 |
Actions (login required)
 |
View Item |
