Sukarne, Sukarne and DEPAMEDE, SULAIMAN N and Amin, Muhamad and Ali, Muhamad Turnitin: Molasses as an Alternative Low-cost Medium for Production of Recombinant vp28-Antigen in Escherichia coli. Mataram University Press.
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Abstract
Previous studies reported that VP28, which is a capsid protein of White Spot Syndrome Virus (WSSV), showed to be a potential candidate for WSSV vaccine. However, the large-scale production of the protein is hampered by the availability and expensive cost of culture media. Thus, this study was aimed at finding alternative low-cost medium for growing bacterial hosts carrying plasmid for producing VP28 proteins fused with glutathione s-transferase (GST) enzyme which then indicated as GST.VP28 fusion protein. The result showed that 7.5% molasses seemed to have better growth than commercial media (LB). Other result also indicated that quality of GST.VP28 protein expressed by host in 7.5% molasses appeared to be good. Ass indicated by its solubility. This result suggested that molasses medium can be an alternative low-cost medium for large-scale production of GST.VP28 protein
| Item Type: | Other |
|---|---|
| Keywords (Kata Kunci): | white spot syndrome virus (WSSV), VP28, molasse |
| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Fakultas Peternakan |
| Depositing User: | . Zulkarnain Zulkarnain |
| Date Deposited: | 11 Feb 2021 00:38 |
| Last Modified: | 11 Feb 2021 00:38 |
| URI: | http://eprints.unram.ac.id/id/eprint/20909 |
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