Development Of Enzyme-Linkage Immunosorbent Assay Against Type B Of Clostridium Botulinum: A Preliminary Study

Depameda, Sulaiman, N. (2011) Development Of Enzyme-Linkage Immunosorbent Assay Against Type B Of Clostridium Botulinum: A Preliminary Study. Development Of Enzyme-Linkage Immunosorbent Assay Against Type B Of Clostridium Botulinum: A Preliminary Study, 36 (4). pp. 237-242. ISSN 2460-6278

[img]
Preview
 Text
DEVELOPMENT OF ENZYME-LINKAGE IMMUNOSORBENT ASSAY.pdf
Download (1MB) | Preview
Official URL: https://ejournal.undip.ac.id/index.php/jitaa/artic...

Abstract

Clostridium botulinum neurotoxin (BoNTs) is one of the causes of economic loss in the livestock industry. This economic loss would be as a direct result when animals poisoned by BoNTs or indirectly when the livestock products are contaminated by BoNTs, which end up with the products are banned by authority. Therefore a routine surveillance of BoNTs in the farm and in livestock product processing industry is urgently needed. One of the most relatively quick and accurate methods to perform a routine detection of the presence of BoNTs is enzyme-linkage immunosorbant assay (ELISA). In this article we describe the results of the development of ELISA, using polyclonal antibodies against BoNTs-B produced locally. Antibodies were generated from six Balb/c mice with standard immunological methods. Mice were immunized three times for a period of 8 weeks with a commercial type B Clostridium botulinum toxoid at a dose of 100 ng per mouse per injection. The resulting antibody was purified by a combination of ammonium sulfate precipitation 50% (w/v) technique and a protein A column method. The results of this preliminary study indicated that the developed ELISA method capable of detecting type B Clostridium botulinum toxin up to 1.0 ng/ml.

Item Type: Article
Keywords (Kata Kunci): C. botulinum toxin, ELISA, polyclonal antibody
Subjects: S Agriculture > SF Animal culture
Divisions: Fakultas Peternakan
Depositing User: Ir Djoko Kisworo, M.Sc., Ph.D
Date Deposited: 13 Oct 2021 00:43
Last Modified: 13 Oct 2021 00:43
URI: http://eprints.unram.ac.id/id/eprint/24918

Actions (login required)

View Item View Item
Repository UNRAM is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.