Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate

DEPAMEDE, SULAIMAN N and Djoko, Kisworo and Asri, Niken (2012) Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate. Fakultas Peternakan. (Unpublished)

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Abstract

Abstract Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 µg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 µg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 µg/ml isolated lysozyme were comparable to 800 µg/ml of a commercial hen egg white lysozyme.

Item Type: Other
Keywords (Kata Kunci): Key words: Lysozyme, Bali cattle, Bos sondaicus, PEG, aqueous mixture system
Subjects: S Agriculture > SF Animal culture
Divisions: Fakultas Peternakan
Depositing User: Ir Djoko Kisworo, M.Sc., Ph.D
Date Deposited: 20 Oct 2021 23:45
Last Modified: 20 Oct 2021 23:45
URI: http://eprints.unram.ac.id/id/eprint/25154

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