DEPAMEDE, SULAIMAN N and Djoko, Kisworo and Asri, Niken
(2012)
Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate.
Fakultas Peternakan.
(Unpublished)
Abstract
Abstract
Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 µg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 µg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 µg/ml isolated lysozyme were comparable to 800 µg/ml of a commercial hen egg white lysozyme.
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Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate. (deposited 20 Oct 2021 23:45)
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