Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development

Djoko, Kisworo and DEPAMEDE, SULAIMAN N (2021) Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development. Fakultas Peternakan. (Unpublished)

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Abstract

Objective: The present study aimed to produce and analyze antibody against a synthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva. Materials and Methods: The synthetic amino acid sequence of the PIP-HP (VIRELGICPDDWAVIPIKANRF) was developed, conjugated to bovine serum albumin and was used to immunize Indonesian local rabbits. Positive sera that specific against the PIP-HP were pooled and purified sequentially by implying ammonium sulfate precipitation and protein A affinity methods. Purified antibody was then employed to analyze of PIP-HP in the ruminants’saliva by means of westernblot assays. Results: A polyclonal antibody specific to asynthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva was successfully produced. Our results show that the antibody potentially to be used to develop an immuno-diagnostic kit. Furthermore, the antibody was also able to inhibit the growth of both Escherichia coli and Staphylococcus aureus cultures significantly (P<0.001) at concentration of 0.3 mg/mL. Conclusions: In this study, antibody against synthetic peptide of PIP-HP (VIRELGICPDDWAVIPIKANRF) of Bali cattle saliva origin has been produced successfully. Further studies need to be carried out since the antibody has potential as biomarker for non-invasive immunoassays development.

Item Type: Other
Keywords (Kata Kunci): Amino acid; Bactericidal; E. coli; Innate immunity; Lateral flow assay; S. aureus
Subjects: S Agriculture > SF Animal culture
Divisions: Fakultas Peternakan
Depositing User: Ir Djoko Kisworo, M.Sc., Ph.D
Date Deposited: 10 Nov 2021 00:15
Last Modified: 10 Nov 2021 00:15
URI: http://eprints.unram.ac.id/id/eprint/25633

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