Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate

Depamede, S N and Asri, N and Julisaniah, N I and Suryadi, B F and Kisworo, D (2012) Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate. Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate, 11 (8). pp. 1977-1980. ISSN 1684–5315

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Abstract

Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 µg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 µg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 µg/ml isolated lysozyme were comparable to 800 µg/ml of a commercial hen egg white lysozyme.

Item Type: Article
Keywords (Kata Kunci): Lysozyme, Bali cattle, Bos sondaicus, PEG, aqueous mixture system.
Subjects: Q Science > QL Zoology
Divisions: Fakultas Peternakan
Depositing User: Dr. Bambang Fajar Suryadi
Date Deposited: 15 Dec 2021 04:25
Last Modified: 15 Dec 2021 04:25
URI: http://eprints.unram.ac.id/id/eprint/26492

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