Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus

Suryadi, B F and Yanuwiadi, B and Ardyati, T and Suharjono, S (2015) Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus. International Journal of Microbiology, 2015 (854709). pp. 1-6. ISSN 16879198, 1687918X

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Malaria is endemic to Lombok Island, Indonesia. One approach to suppress malaria spread is to eliminate anopheline larvae in their habitat and the environmentally safe agent is bacteria, that is, Bacillus sphaericus. However, there is no information regarding local isolate of B. sphaericus thatistoxictomosquitolarvaefromLombok.heaimsofthestudyweretoisolate B. sphaericus from soil in areas close to beach surrounding Lombok Island and to test their toxicity against 3rd instar Anopheles aconitus larvae. Soil samples were collected from 20 diferent sampling locations from Lombok Island and homogenized with sterile physiological salt solution. Suspension was heat-shocked at 80∘ C for 30 minutes and then spread onto antibiotic-supplemented NYSM solid medium. Colonies grown were characterized and subjected to initial toxicity test against anopheline larvae. Isolates with more than 50% killing percentage were subjected to bioassay testing against anopheline larvae. From 20 locations, 1 isolate showed mild toxicity (namely, isolate MNT) and 2 isolates showed high toxicity (namely, isolates SLG and TJL2) against An. aconitus.hose3 isolates were potentially useful isolates, as they killed almost all larvae in 24 hours.he discovery of toxic indigenous isolates of B. sphaericus from Lombok Island opens opportunity to develop a biopesticide from local resources.

Item Type: Article
Keywords (Kata Kunci): Bacillus sphaericus, Anopheles aconitus, Lombok Island
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Matematika dan ilmu Pengetahuan Alam
Depositing User: Dr. Bambang Fajar Suryadi
Date Deposited: 15 Dec 2021 04:25
Last Modified: 15 Dec 2021 04:25

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