Novel SNP of Calpain-1 (CAPN1) Gene and Its Association with Carcass and Meat Characteristics Traits In Bali Cattle

Pratiwi, N. and Maskur, Maskur and Priyanto, R. and Jakaria, Jakaria (2016) Novel SNP of Calpain-1 (CAPN1) Gene and Its Association with Carcass and Meat Characteristics Traits In Bali Cattle. Journal of the Indonesian Tropical Animal Agriculture, 41 (3). pp. 109-116. ISSN 2087-8273 eISSN 2460-6278

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Abstract

Calpain-1 gene (CAPN1) produces an calpain enzyme controlling structure of meat protein and tenderness. The aims of this study were to identify Single Nucleotide Polymorphism (SNP) in exon 5 and 6 of CAPN1 gene and its associate with carcass and meat characteristic traits in bali cattle. A total of 48 bali cattles from BPTU-HMT Bali Cattle, Bali Province were used in the research. SNP in exon 5 and 6 of CAPN1 gene were identify with direct sequencing using MEGA 5 program. Analysis of polymorphism was conducted by PopGen 1.32 software to identify frequencies of genotype, allele and Hardy-Weinberg equilibrium. The association of CAPN1 gene genotype with carcass and meat characteristic traits was analyzed using Generalized Linear Model (GLM) procedure of SAS. Sequencing analysis at exon 5-6 of CAPN1 gene in Bali cattle resulted in eight polymorphic SNPs. They are c.3669T>C, c.3854G>A, c.3881T>C, c.3899C>T, c.3908C>G, c.4002C>A, c.4021G>T and c.4037A>C. The SNPs c.3669T>C, c.3854G>A and c.3899C>T were significantly (P<0.05) associated with rump thickness (RT), rump fat thickness (RFT) and marbling score (MS), while SNP c.4037A>C was not significantly associated with carcass and meat characteristic traits. The SNPs were significantly associated with carcass and meat characteristic traits namely c.3669T>C, c.3854G>A and c.3899C>T. Those SNPs may be used as candidate marker for Marker Assisted Selection (MAS) in bali cattle.

Item Type:	Article
Keywords (Kata Kunci):	Bali cattle, CAPN1 gene, SNP
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Peternakan
Depositing User:	Relanti Saleha
Date Deposited:	26 Dec 2022 05:54
Last Modified:	26 Dec 2022 05:54
URI:	http://eprints.unram.ac.id/id/eprint/33804

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