Listiana, Baiq Erna and Sumarjan, Sumarjan and Schurr, Ulrich and Mulyaningsih, Tri IN VITRO REGENERATION OF AGARWOOD PLANT (Aquilaria filarial). In: The 3rd International Conference on Science and Technology 2018, 10 December 2018, Mataram, Lombok.
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Abstract
Aquilaria filaria is one of the species producing fragrant oleoresin agarwood, which is endemic to Eastern of Indonesia. In 2004, CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) incorporated A. filaria as a vulnerable species due to the declining population of this tree in its native habitat. Plant in vitro culture is only a strategy to propagate plant material in prolific rates for in situ and ex situ germplasm preservation in a range of environmental condition. This research aims to study de novo regeneration of shoot and root in plant tissue culture of A. filaria for in vitro preservation of this vulnerable plant species. In vitro cultivation conducted in Murashige and Skoog (MS) medium containing phytohormone auxin: indole-3-butyric acid (IBA), α-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) and cytokinin: 6-benzylaminopurine (BAP) and kinetin. The result reveals that leaf is a suitable material for callus induction. High proliferating callus is induced in medium containing combination of IBA (2 mg/l) or NAA (0.2 – 1 mg/l) with cytokinin BAP (0.5 – 1 mg/l). A. filaria plant callus regenerate shoot primordia by an addition of single BAP (0.5 mg/l) or combination with IBA or NAA (0.2 mg/l) into the culture medium. Rooting of shoot cultures was achieved in medium with addition of a sole phytohormone IBA (1 – 2 mg/l) or NAA (0.2 – 2 mg/l). The result of this study provides basis for in vitro propagation of A. filaria and conservation of agarwood germplasm.
| Item Type: | Conference or Workshop Item (Paper) |
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| Keywords (Kata Kunci): | germplasm conservation, plant tissue culture, phytohormone |
| Subjects: | Q Science > QK Botany |
| Divisions: | Fakultas Matematika dan ilmu Pengetahuan Alam |
| Depositing User: | Dr Tri Mulyaningsih |
| Date Deposited: | 12 Apr 2023 03:11 |
| Last Modified: | 12 Apr 2023 03:11 |
| URI: | http://eprints.unram.ac.id/id/eprint/36154 |
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