JURNAL - MACROALGAE BIOACTIVE COMPOUNDS FOR THE POTENTIAL ANTIVIRAL OF SARS-COV-2: AN IN SILICO STUDY

Padmi, Hasriaton and Kharisma, Dhea Viol and Ansori, Arif Nur Muhammad and Sibero, Mada Triandala and Widyananda, Muhammad Hermawan and Ullah, Md. Emdad and Gumenyuk, Olga and Chylichcova, Svetiana and Bratishko, Natalia and Prasedya, Eka Sunarwidhi and Sucipto, Teguh Hari and Zainul, Rahadian (2022) JURNAL - MACROALGAE BIOACTIVE COMPOUNDS FOR THE POTENTIAL ANTIVIRAL OF SARS-COV-2: AN IN SILICO STUDY. Journal of Pure and Applied Microbiology, 16 (2). pp. 1018-1027. ISSN 1018-1027

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Official URL: https://microbiologyjournal.org/

Abstract

Functionalization of nanocarriers has been considered the most promising way of ensuring an accurate and targeted drug delivery system. This study reports the synthesis of bifunctional folic-conjugated aspartic�modified Fe3O4 nanocarriers with an excellent ability to deliver doxorubicin (DOX), an anticancer drug, into the intercellular matrix. Here, the presence of amine and carboxylate groups enables aspartic acid (AA) to be used as an efficient anchoring molecule for the conjugation of folic acid (FA) (EDC–NHS coupling) and DOX (electrostatic interaction). Based on the results, surface functionalization showed little effect on the physicochemical properties of the nanoparticles but significantly influenced both the loading and release efficiency of DOX. This is primarily caused by the steric hindrance effect due to large and bulky FA molecules. Furthermore, in vitro MTT assay of B16–F1 cell lines revealed that FA conjugation was responsible for a significant increase in the cytotoxicity of DOX-loaded nanocarriers, which was also found to be proportional to AA concentration. This high cytotoxicity resulted from an efficient cellular uptake induced by the over-expressed folate receptors and fast pH triggered DOX release inside the target cell. Here, the lowest IC50 value of DOX-loaded nanocarriers was achieved at 2.814 � 0.449 mg mL�1 . Besides, further investigation also showed that the drug-loaded nanocarriers exhibited less or no toxicity against normal cells

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Matematika dan ilmu Pengetahuan Alam
Depositing User: Mr Eka Sunarwidhi Prasedya
Date Deposited: 12 Apr 2023 07:28
Last Modified: 12 Apr 2023 07:28
URI: http://eprints.unram.ac.id/id/eprint/36188

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