Sunarwidhi, Anggit Listyacahyani and rosyantari, Andhia and Prasedya, Eka Sunarwidhi and Ardiana, Nur and Ilhami, Bq Tri Khairina and Abidin, Angga Susmana and Ambana, Yogi and Kirana, Indah Alibiah Putri and Wirasisya, Dyke Gita and Anugrah, Windah and Fersiyana, Rizqa Deccati and Dewi, Ni Made Amelia Ratnata (2021) Similarity Check - The correlation between total protein content and antioxidant activity of collagen isolated from a marine sponge Stylissa flabelliformis collected from North Lombok Indonesia coast. IOP Conference Series: Earth and Environmental Science. ISSN 1755-1315
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Abstract
Collagen is a fibrous protein that has recently gained high attention from the pharmaceutical industry due to its benefits on the skin. Collagen can be isolated from various resources including marine sponges. Marine sponges are found in a large amount in Indonesia and has not been widely explored for its pharmacology benefits. Here we isolate collagen from a marine sponge Stylissa flabelliformis found in North Lombok Indonesia coast. The isolation of collagen was performed followed by total protein content analysis using modified Bradford protein assay and antioxidant activity measurement using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The total yield of the collagen isolate obtained was 3.5% and it had a total of 0.755mg/ml protein. DPPH assay has shown that the collagen isolate had antioxidant activity with an IC50 of 61.5±2.132 ppm. Based on Spearman correlation assay, the antioxidant activity was found to be correlated with the protein content of the isolate (r value=0.8). These results show the potency of using the collagen isolated from marine sponge Stylissa flabelliformis for further antioxidant benefits.
| Item Type: | Article |
|---|---|
| Keywords (Kata Kunci): | Marine collagen, Marine sponge, Total protein, Antioxidant |
| Subjects: | A General Works > AI Indexes (General) |
| Divisions: | Program Studi Farmasi |
| Depositing User: | Mr Eka Sunarwidhi Prasedya |
| Date Deposited: | 12 Apr 2023 14:20 |
| Last Modified: | 12 Apr 2023 14:20 |
| URI: | http://eprints.unram.ac.id/id/eprint/36209 |
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