Induction of Agarwood Olfactory Sesquiterpenesfrom Aquilaria filaria Callus Culture

Listiana, Bq. E. and Sumarjan, Sumarjan and Mulyaningsih, Tri and Roloff, M. and Schurr, Ulrich (2021) Induction of Agarwood Olfactory Sesquiterpenesfrom Aquilaria filaria Callus Culture. ASM Sc. Journal, 14 (2). pp. 127-135. ISSN 2682-8901

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Abstract

Aquilaria filaria is a native tree species from the East Indonesian forests, whose heartwood producesan aromatic resin which is widely used as the fragrance raw material. This oleoresin known as“agarwood” is rich in unique olfactory molecules and exhibits great industrial demands with highmarket values globally. However, as a consequence of non-sustainable harvesting in the past, A. filariahas been included in the IUCN (International Union for Conservation of Nature) red list of threatenedspecies. In an effort to meet the demand for the fragrance compounds from the valuable agarwoodusing plant biotechnology, this research aimed to evaluate the potential of A. filaria callus culture toproduce volatile fragrance sesquiterpenes (SQTs) under the stimulation of selected biotic and abioticelicitors. SQTs emitted from the callus cultures were extracted using a stir bar sorptive extraction(SBSE) method and the components were identified using GC-MS. The results revealed that theproduction of sixteen fragrance SQTs previously identified as the olfactory compounds of agarwoodwere highly inducible in in vitro A. filaria cultures. Some of those agarwood compounds were jinkohol,acorenone, α-agarofuran, dehydrojinkoh-eremol, spathulenol, α-guaiene, and α-cedrene. It wasconcluded that callus cultures of A. filaria have the potential to be developed as a bioreactor for theagarwood fragrance compounds production

Item Type: Article
Keywords (Kata Kunci): callus culture; fragrance oleoresin; SBSE; sesquiterpenes
Subjects: Q Science > QK Botany
Divisions: Fakultas Matematika dan ilmu Pengetahuan Alam
Depositing User: Dr Tri Mulyaningsih
Date Deposited: 13 Apr 2023 00:31
Last Modified: 13 Apr 2023 00:31
URI: http://eprints.unram.ac.id/id/eprint/36248

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