Suaib Suaib, Suaib Suaib and Norma Arief, Norma Arief and Gusti Ray Sadimantara, Gusti Ray Sadimantara and Ni Wayan Sri Suliartini, Ni Wayan Sri Suliartini and Tresjia Corina Rakian, Tresjia Corina Rakian and Tresjia Corina Rakian, Tresjia Corina Rakian and Ardhi, Ardhi (2018) In vitro Seeds Germination and Plantlets Growth of Hot Pepper (Capsicum frutescens L.) On Non-autoclaved Murashige and Skoog basal Medium. Asian Journal of Plant Sciences, 17 (4). pp. 173-181. ISSN 1682-3974
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In vitro Seeds Germination and Plantlets Growth of Hot Pepper (Capsicum frutescens L.) On Non-autoclaved Murashige and Skoogbasal Medium.pdf Download (3MB) | Preview |
Abstract
Background and Objective: In traditional plant tissue culture, the higher cost due to the expensive laminar air flow cabinet and autoclave. Time consuming in lengthy procedures of implementation and excessive electrical power due to unexpected power outage were the main obstacles in the success of modern plant tissue culture. By these obstacles, the use of active chlorine (NaClO) in the MS basal medium on effectiveness of contamination, seed germination and plantlet growth of hot pepper ( Capsicum frutescens L.) in vitro culture was the objective of the research. Materials and Methods: Hot pepper seeds and MS basal medium were used as experimental materials and sterilized by 5.25% active chlorine contained in commercial household bleach in 13 various percentages of treatments ranged from 0.079-0.394% and arranged in completely randomized design (CRD). Observed variables were percentage of medium contamination, germination seed rate and percentage, plant height, leaf number, root length and root number. Results: The study indicated that the percentage of jar contamination during 30 days of incubation were 4 of 5 jars cultured or 80% in NaClO lower concentration (0.079%), whereas, in higher concentration (0.394%) was 1 of 5 jars cultured or 20%. Both of these concentrations had the same effect in the early of seed germination i.e., 2 days after incubation. The highest plant height was achieved in 0.394% and the highest number of leaves was noted in 0.368%, also the highest root length was in 0.105%. The root number was not of different significantly for all concentrations which range from 13.20-22.70. Conclusion: Hot pepper seeds germination and plantlets growth had successfully been achieved under non-autoclaved in vitro medium.
| Item Type: | Article |
|---|---|
| Keywords (Kata Kunci): | Active chlorine, medium contamination, hot pepper, seed germination, plantlet growth |
| Subjects: | S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture S Agriculture > SH Aquaculture. Fisheries. Angling |
| Divisions: | Fakultas Pertanian |
| Depositing User: | Dr. Ni Wayan Sri Suliartini, SP., MP |
| Date Deposited: | 16 Apr 2023 00:53 |
| Last Modified: | 16 Apr 2023 00:53 |
| URI: | http://eprints.unram.ac.id/id/eprint/36490 |
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