Ekpresi Protein Spesifik Embrio Praimplantasi Kambing Secara in Vitro Sebagai Sinyal Kebuntingan Dini (Specific Protein Exspression of Goat Preimplantation Embryo in Vitro Asanearly Pregnanci sigenal ). (Jurnal Veteriner. Jurnal

Sumadiasa, I Wayan Lanus and Yuliani, Enny (2008) Ekpresi Protein Spesifik Embrio Praimplantasi Kambing Secara in Vitro Sebagai Sinyal Kebuntingan Dini (Specific Protein Exspression of Goat Preimplantation Embryo in Vitro Asanearly Pregnanci sigenal ). (Jurnal Veteriner. Jurnal. urnal Veteriner. Jurnal Kedokteran Hewan Indonesi Kedokteran Hewan Indonesia. Vol.9,N0.2. ),1411 - 8327. Ketua wayan L S, Enny Yuliani, Terakreditasi Dirjen Dikti, SK.nomor. 55/DIKTI/KEP/2005, hal. 79-86, 9 (2). pp. 79-86. ISSN 2477-5665.

Preview	Text Ekspresi protein.pdf Download (749kB) | Preview
Preview	Text ekpresi protein spesifik embrio praimplantasi kambing secara in vitro sebagai sinyal kebuntingan dini.pdf Download (2MB) | Preview
Preview	Text REV 1 & 2. EKSPRESI PROTEIN.pdf Download (994kB) | Preview

Abstract

he aim of the experiment was to investigate the intensity of signal secreted by embryos for their survival in the early pregnancy. Embryos at morulla and blastocyst stages generated by in vitro fertilization were frozen and then thawed for in vitro culture in tissue culture medium. The protein secreted by embryo were detected 48 hours after thawing for those frozen at morulla stage, and 24 hours and 72 hours after thawing for those frozen in the blastocyst stage. Results showed that preimplantation goat embryos secreted an average of 3,041 µg protein per ml medium for the embryo cultured in tissue culture medium (TCM) 199 + 0,1 % BSA and 1,992 µg protein per ml medium for embryo cultured in TCM without BSA. Non growing embryos secreted only a small amount protein, i.e. 0,434 µg protein per ml medium and 0,417 µg protein per ml medium respectively for embryo cultured TCM 199 with BSA and TCM without BSA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed protein bands with many different molecular weight of 100, 95 , 55 , 43 , 28 and 18 KD. It appeared that the growing embryos secreted more proteins as compared to those non growing embryos.

Item Type:	Article
Keywords (Kata Kunci):	protein spesipik, embrio kambing, in Vitro
Subjects:	Q Science > QL Zoology
Divisions:	Fakultas Peternakan
Depositing User:	Mayadi
Date Deposited:	19 May 2023 06:02
Last Modified:	19 May 2023 06:02
URI:	http://eprints.unram.ac.id/id/eprint/38158

Actions (login required)

View Item