Sumadiasa, I Wayan Lanus and SUSILAWATI, TRINIL and CIPTADI, GATOT and ISNAINI, NURUL (2015) The potency of guava filtrate (Psidium guajava Linn) for preservation of Bali bull spermatozoa. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS), 8 (5). pp. 51-57. ISSN e-ISSN: 2319-2380, p-ISSN: 2319-2372.
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The potency of guava filtrate (Psidium guajava Linn) for preservation of Bali bull spermatozoa.pdf Download (2MB) | Preview |
Abstract
The aim of this research was to investigate the potency of guava filtrate (GF) in synthetics cauda epididymal plasma (CEP-2) based diluent to maintaining the quality of Bali bull spermatozoa during chilled storage (5º C). Ten ejaculates from Bali bulls were collected using an artificial vagina once a week. Each ejaculate was divided into seven aliquots, each aliquot diluted with CEP-2 diluents containing 0%, 4%, 6%, 8%, 10%, 12% and 14% GF (v/v), respectively, to replace 20% egg yolk (EY) in the diluent. All samples were placed in the refrigerator and cooled gradually to 5º C. Once reached 5º C, they were kept at this temperature and stored for 8 days. The mean percentages of progressive motility and viability of spermatozoa stored for 8 days in diluent containing 10% GF was significantly higher (p<0.05) than other six diluents containing different levels of GF. Conversely, the percentage of abnormal spermatozoa was significantly lower (p<0.05) in the previous diluent compared with the latter ones. In conclution, guava filtrate is potential when included in CEP�2 diluent for maintaining the quality of Bali bull spermatozoa at 5º C. It seems that 10% GF is the best level for substituting 20% EY.
| Item Type: | Article |
|---|---|
| Keywords (Kata Kunci): | Bali bull, chilled storage, preserve, guava diluent, semen quality |
| Subjects: | Q Science > QL Zoology |
| Divisions: | Fakultas Peternakan |
| Depositing User: | Dr I Wayan Lanus Sumadiasa |
| Date Deposited: | 15 Jun 2023 03:59 |
| Last Modified: | 15 Jun 2023 03:59 |
| URI: | http://eprints.unram.ac.id/id/eprint/38168 |
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