Development of a rapid immunochromatography test for detecting antibodies after anthrax vaccination in cattle: A preliminary study

Made Sriasih, Sriasih and NGONGU DEPAMEDE, SULAIMAN and Rosyidi, Anwar and Yulianti, Enny (2015) Development of a rapid immunochromatography test for detecting antibodies after anthrax vaccination in cattle: A preliminary study. Proceeding of the 5th International Conference on sustainable Agriculture for developing Countries.. pp. 32-35. ISSN ISBN 978-602-8475-87-7

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Abstract

nthrax is a zoonotic disease of strategic importance with high mortality rate in ruminants. Vaccination and monitoring of antibody titer after anthrax vaccination have been carried out in an effort to prevent anthrax disease in cattle. Enzyme linked immunosorbent assay (ELISA) is a method commonly used in the monitoring of antibody titer after vaccination. However, the use of the ELISA method requires skilled laboratory personnel, specialized laboratory equipment, and relatively more expensive. This study aimed to develop a method to detect antibodies after anthrax vaccination in cattle using rapid immunochromatography detection. Colloidal gold as a marker were conjugated with protective antigen (PA), with a concentration of 0.2, 0.4 and 0.8mg/ml, then put on the conjugate pads as part of the immunochromatography test strip. A total of 13 serum samples of cattle after vaccination was used in this study. The sample consisted of seven positive and six negative sera samples based on the results of the ELISA test to detect the presence or absence of anti-PA antibodies in serum. The results using the rapid immunochromatography test indicate that the anti-PA antibodies in the serum can be detected within 10min. Antigen concentration of 0.2mg/ml in the conjugate pads showed the same sensitivity test as other antigen concentrations used in this study.

Item Type: Article
Keywords (Kata Kunci): anthrax, rapid test, immunochromatography, cattle, protective-antigen
Subjects: Q Science > QR Microbiology > QR180 Immunology
Divisions: Fakultas Peternakan
Depositing User: Dr. drh. Anwar Rosyidi .MP
Date Deposited: 12 Jun 2023 08:33
Last Modified: 12 Jun 2023 08:33
URI: http://eprints.unram.ac.id/id/eprint/39708

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