Ilmi, Nurul (2018) Molecular Identification of Endophytic Bacteria from The Stem’s Bark of Moringa Plant and Their Antibacterial Activities. S1 thesis, Universitas Mataram.
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Abstract
Moringa oliefera is one of the plants that is scientifically proven to contain secondary metabolites of flavonoids, alkaloids, phenols that can inhibit bacterial activity. Extraction of bioactive compounds from plants is considered inefficient because it requires a large biomass. The effective method is by utilizing endophytic bacteria associated with the plant. The aim of this research were to know the ability of endophytic bacterial isolate of bark of moringa plants in inhibiting the growth of gram-positive and gram-negative bacteria, and to characterize the biochemical and morphologycal properties of the isolated endophytic bacteria, and to determine species of endophytic bacterial isolate based on 16S rRNA. The stage of this research were endophytic bacterial isolation, antibacterial activity test, biochemical and morphological characterization, and molecular identification of the endophytic bacteria isolates based on 16S rRNA barcoding. Four isolates (KK1, KK2, KK3 and KK4) endophytic bacteria were isolated from the bark of moringa stems. Antibacterial activity test, showed that 4 endophytic bacterial isolates were able to inhibit the growth of B. cereus, S. aureus, E. coli and S. marcescen. Based on molecular identification with 16S rRNA, endophytic bacterial isolates of moringa plant stems was very closely related to B. cereus JL., and B. cereus strain ATCC 14579.
| Item Type: | Thesis (S1) |
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| Keywords (Kata Kunci): | antibacterial activity, endophytic bacteria, stem of moringa plant, 16S rRNA |
| Subjects: | L Education > L Education (General) |
| Divisions: | Fakultas Keguruan dan Ilmu Pendidikan |
| Depositing User: | Wiwin Kartikawati |
| Date Deposited: | 25 Oct 2018 02:07 |
| Last Modified: | 25 Oct 2018 02:07 |
| URI: | http://eprints.unram.ac.id/id/eprint/9122 |
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