Isolation and Characterization of Excretory/Secretory Antigenic Proteins of Adult Fasciola gigantica Lombok Isolate

Ali, Muhamad and Made Sriasih, Sriasih and Sulaiman N. Depamede, Sulaiman Isolation and Characterization of Excretory/Secretory Antigenic Proteins of Adult Fasciola gigantica Lombok Isolate. In: Sustainable Livestock Production in the Perspective of Food Security, Policy, Genetic Resources, and Climate Change.

[img] Text
2. SI-01 Isolation and caharcterization .pdf

Download (4MB)

Abstract

The objective of this study was to isolate excretory/secretory (ES) proteins of adult Fasciola gigantica local isolate (Lombok) and to determine its antigenic proteins using Western blotting. The ES components were isolated from live adult F. gigantica collected from liver of Fasciolosis positive Bali cattle slaughtered at the abattoir. Isolated ES were then characterized using 1-D gel electrophoresis and Western blotting. Gel electrophoresis analysis showed that the ES components of F. gigantica Lombok isolate consist of proteins with a molecular weight of 7 to 25 kDa. Western blotting analysis using sera of Fasciolosis positive Bali cattle (n = 2) exhibited several antigenic proteins with a molecular weight of 47.7, 25, 21.4 to 17.9, 16.4 and 10.5 to 8.05 kDa. Amongst the proteins detected, the 25 kDa was sharply reacted in both positive sample. This study suggests that the proteins recognised are an immunologically active component of the ES and antibodies to those proteins were present in naturally infected cattle. The results of this study are expected to support the Fasciolosis control program in cattle through a specific early detection and vaccination.

Item Type: Conference or Workshop Item (Paper)
Keywords (Kata Kunci): Fasciolosis, Bali cattle, 1-D gel electrophoresis, Western blotting, Antigenic proteins
Subjects: Q Science > Q Science (General)
S Agriculture > SF Animal culture
Divisions: Fakultas Peternakan
Depositing User: . Zulkarnain Zulkarnain
Date Deposited: 06 Nov 2020 03:41
Last Modified: 06 Nov 2020 03:41
URI: http://eprints.unram.ac.id/id/eprint/18235

Actions (login required)

View Item View Item