Cloning, expression, and functional assay of monoclonal antibody fab fragment against merozoite surface protein 1 (msp1) of Plasmodium falciparum.

Yunita Sabrina, Yunita and Muhamad Ali, Muhamad Ali (2013) Cloning, expression, and functional assay of monoclonal antibody fab fragment against merozoite surface protein 1 (msp1) of Plasmodium falciparum. Journal of Bioscience Research, 10 (2). pp. 71-77. ISSN 18119506, 22183973

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Abstract

The cloning of antibody-encoding genes is a pivotal role for generation antibody variants with a higher affinity and a lower immunogenicity. This study was successfully cloned a fab fragment of monoclonal antibody against Merozoite Surface Protein 1 (MSP1) of Plasmodium falciparum from antibody-producing hybridoma and expressed in E. coli cell-free expression system. ELISA result showed that the generated antibody could bind with their antigen (MSP119) in specifically manner. Addition of DsbC, periplasmic disulfide bond isomerase, gave a higher ELISA signal, indicating that the addition of DsbC accelerated disulfide isomerization in the expression system resulted in proper folding of the generated antibody. The sequence analysis of the fab fragment genes consists of 663 nucleotide of Light Chain (LC) and 645 nucleotides of Heavy Chain (HC). The final products of this research are not only active antibodies but also the antibodies-encoding gene, which allows further engineering to produce the antibody variants with desired properties and applications.

Item Type: Article
Keywords (Kata Kunci): Monoclonal antibody, Fab fragment, Merozoite Surface Protein 1 (MSP1), Plasmodium falciparum
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Peternakan
Depositing User: Ali Muhamad Ali
Date Deposited: 29 Sep 2021 03:34
Last Modified: 29 Sep 2021 03:34
URI: http://eprints.unram.ac.id/id/eprint/24665

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