Characterization of VP28 and VP19-encoding Genes and Protein Sequence of White Spot Syndrome Virus (WSSV) Isolated from Indonesia

Muhamad Ali, Muhamad Ali and Sulaiman N. Depamede, Sulaiman and Bagus DH Septiono, Bagus DH Septiono and Alis Mukhlis, Alis Mukhlis and Sahrul Alim, Sahrul Alim and Muhamad Amin, Muhamad Amin and Muhamad Ashari, Muhamad Ashari (2015) Characterization of VP28 and VP19-encoding Genes and Protein Sequence of White Spot Syndrome Virus (WSSV) Isolated from Indonesia. International Seminar on Tropical Natural Resouces (ISTNR 2015), 1 (1). pp. 1-12. ISSN 978-979-8911-85-9

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Abstract

This study was aimed to compare VP19 and VP28 genes of WSSV isolated from Situbondo, Indonesia to other WSSV strains isolated from 12 different countries. The result indicated that VP19 gene from Situbondo appeared to be very uniqe. The nucleotide sequence of VP19 gene showed 100% identical to none of those 12 VP19 genes of WSSV. The nucleotide differences were observed initially at nucleotide position of 54, 108, 162, 201, and 218 nt. Meanwhile, VP28 gene of Situbondo appeared to be quite conserved. Compared to those 12 other WSSV strains, VP28 gene sequence of Situbondo showed 100% identical to VP28 gene sequence of WSSV isolated from China/Qindao, Japan, Korea01, South Carolina (USA) and also previous indonesian isolate (Indonesia97). However, the VP28 gene showed also some nucleotide differences compared to VP28 gene from China/Dalian, India, Iran, Korea Mexico, Thailand and Vietnam/Ho Chi Minh. The similarity was recorded at 99.4%, and the nucleotide differences were found at position 32, 255, 347, 396, 398, 430 and 458 nt. The variability in VP19 gene suggested that WSSV from Situbondo was quite different than the previous WSSV strain isolated from other region of Indonesia. However, based on VP28 gene, Situbondo isolate appeared to be 100% identical to previous Indonesia isolate. This result could be an important clue that VP28 gene is a conserved gene from which WSSV vaccine could be potentially developed.

Item Type:	Article
Keywords (Kata Kunci):	WSSV, VP28, VP19, homology.
Subjects:	Q Science > QR Microbiology
Divisions:	Fakultas Peternakan
Depositing User:	Ali Muhamad Ali
Date Deposited:	30 Sep 2021 00:30
Last Modified:	30 Sep 2021 00:30
URI:	http://eprints.unram.ac.id/id/eprint/24687

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