DEPAMEDE, SULAIMAN N and Djoko, Kisworo and Asri, Niken (2012) Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate. Fakultas Peternakan. (Unpublished)
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Abstract
Abstract Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 µg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 µg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 µg/ml isolated lysozyme were comparable to 800 µg/ml of a commercial hen egg white lysozyme.
| Item Type: | Other |
|---|---|
| Keywords (Kata Kunci): | Key words: Lysozyme, Bali cattle, Bos sondaicus, PEG, aqueous mixture system |
| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Fakultas Peternakan |
| Depositing User: | Ir Djoko Kisworo, M.Sc., Ph.D |
| Date Deposited: | 10 Nov 2021 00:16 |
| Last Modified: | 10 Nov 2021 00:16 |
| URI: | http://eprints.unram.ac.id/id/eprint/25640 |
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Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate. (deposited 20 Oct 2021 23:45)
- Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate. (deposited 10 Nov 2021 00:16) [Currently Displayed]
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