The Use of Natural-Ingredient Medium for Culturing Locally- Isolated Bacillus sphaericus and Its Toxicity Against Anopheles Larvae in Lombok Island, Indonesia

Suryadi, B F and Diarti, W M and Jiwantarum, Y and Zainiati, B L and Pristianingrum, S (2017) The Use of Natural-Ingredient Medium for Culturing Locally- Isolated Bacillus sphaericus and Its Toxicity Against Anopheles Larvae in Lombok Island, Indonesia. In: The 7th Basic Science International Conference, 7-8 Maret 2017, Malang, East Java.

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Abstract

The aim of the study was to explore toxicity of locally-isolated B. sphaericus cultured in natural-ingredient medium (ie. soybean milk, coconut milk and fish flour) widely found in Lombok Island, Indonesia. The bacteria was then assayed against Anopheles larvae naturally- captured from the island. All ingredient were prepared in 30% concentration (v/v or w/v) and used to cultivated B. sphaericus. The cultures were incubated aerobically at 30oC with 180 rpm shaking for 72 hours. Fish flour medium resulted the highest bacterial concentration, followed by coconut milk medium and soybean milk medium was the lowest. However, the endospore concentration percentage was reached by B. sphaericus cultured on coconut milk medium, followed by soybean medium and fish flour medium. The highest mortality rate was showed by B. sphaericus cultured in fish flour medium, followed coconut milk medium and the lowest showed by soybean medium. The highest price was showed by fish flour medium, followed by soybean milk medium and the lowest price was coconut medium. In conclusion, natural-ingredient medium could be used to grow locally- isolated entomopathogenic B. sphaericus to be used for suppressing Anopheles larvae.

Item Type:	Conference or Workshop Item (Paper)
Keywords (Kata Kunci):	Bacillus sphaericus, natural-ingredient medium, Anopheles, larvae and Lombok Island
Subjects:	Q Science > QR Microbiology
Divisions:	Fakultas Matematika dan ilmu Pengetahuan Alam
Depositing User:	Dr. Bambang Fajar Suryadi
Date Deposited:	17 Dec 2021 01:33
Last Modified:	17 Dec 2021 01:33
URI:	http://eprints.unram.ac.id/id/eprint/26564

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