Phenotypic and Genotypic Characterization of Probiotic Bacteria Isolated from Probiotic Dairy Products in the Philippines

Djoko, Kisworo and Barraquio, Virginia, L and Elegado, Francisco, B. (2008) Phenotypic and Genotypic Characterization of Probiotic Bacteria Isolated from Probiotic Dairy Products in the Philippines. Fakultas Peternakan. (Unpublished)

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Abstract

Probiotic products that contain live, beneficial microorganisms that provide health benefits have gained popularity among consumers. Fifteen probiotic bacteria were isolated from probiotic dairy products: Chamyto ® and Yakult ® . All the 14 isolates were Gram positive, catalase negative, rods. One isolate was Gram positive, catalase negative, coccus. The biochemical reaction profiles of 6 selected isolates showed that they were all Lactobacillus paracasei. Their ITS-PCR products showed 65% similarity with Lactobacillus paracasei subsp. paracasei. The 16S rRNA gene sequencing results indicated that the identities of the 3 isolates, 1C10, 2Y9, and 3C10 showed complete (100%) similarity with the 16S rRNA gene of Lb. paracasei. However, 1 isolate, 1Y9, showed 98% similarity with the 16S rRNA gene of Enterococcus faecalis.

Item Type: Other
Keywords (Kata Kunci): Key Words: probiotic, lactic acid bacteria, Lactobacillus paracasei, Enterococcus faecalis
Subjects: S Agriculture > SF Animal culture
Divisions: Fakultas Peternakan
Depositing User: Ir Djoko Kisworo, M.Sc., Ph.D
Date Deposited: 20 Oct 2021 23:45
Last Modified: 20 Oct 2021 23:45
URI: http://eprints.unram.ac.id/id/eprint/25155

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