Prasedya, Eka Sunarwidhi and Padmi, Hasriaton and Ilhami, Bq Tri Khairina and Martyasari, Ni Wayan Riyani and Sunarwidhi, Anggit Listyacahyani and widyastuti, Sri and Khairinisa, Miski Aghnia and Cokrowati, Nunik and Simangunsong, Erika Ernawati and Frediansyah, Andri (2022) JURNAL - BROWN MACROALGAE SARGASSUM CRISTAEFOLIUM EXTRACT INHIBITS MELANIN PRODUCTION AND CELLULAR OXYGEN STRESS IN B16F10 MELANOMA CELLS. Molecules, 27 (23). ISSN 14203049
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Abstract
The brown macroalgae Sargassum has been reported for its anti-UV and photoprotective potential for industrial applications. This study evaluated the melanin inhibition activity of Sar�gassum cristaefolium (SCE) ethanol extract. Melanogenesis inhibition by SCE was assessed in vitro with B16-F10 melanoma cell models and in silico against melanin regulatory proteins Tyrosinase (TYR) and Melanocortin 1 Receptor (MC1R). The regulatory properties evaluated were the melanin content, intracellular tyrosinase activity and cellular antioxidant activities. In addition, the bioactive compounds detected in SCE were subjected to molecular docking against TYR and MC1R. Based on the results, 150 µg/mL SCE effectively inhibited the production of melanin content and intracellular tyrosinase activity. Cellular tyrosinase activity was reduced by SCE-treated cells in a concentration�dependent manner. The results were comparable to the standard tyrosinase inhibitor kojic acid. In addition, SCE effectively decreased the intracellular reactive oxygen species (ROS) levels in B16-F10 cells. The antioxidant properties may also contribute to the inhibition of melanogenesis. In addition, LCMS UHPLC-HR-ESI-MS profiling detected 33 major compounds. The results based on in silico study revealed that the bioactive compound putative kaurenoic acid showed a strong binding affinity against TYR (−6.5 kcal/mol) and MC1R (−8.6 kcal/mol). However, further molecular analyses are needed to confirm the mechanism of SCE on melanin inhibition. Nevertheless, SCE is proposed as an anti-melanogenic and antioxidant agent, which could be further developed into cosmetic skin care products.
| Item Type: | Article |
|---|---|
| Keywords (Kata Kunci): | macroalgae; melanin; melanoma; Sargassum; tyrosinase |
| Subjects: | A General Works > AI Indexes (General) |
| Divisions: | Fakultas Matematika dan ilmu Pengetahuan Alam |
| Depositing User: | Mr Eka Sunarwidhi Prasedya |
| Date Deposited: | 12 Apr 2023 09:45 |
| Last Modified: | 12 Apr 2023 09:45 |
| URI: | http://eprints.unram.ac.id/id/eprint/36204 |
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