Phenotypic and Genotypic Characterization of Probiotic Bacteria Isolated from Probiotic Dairy Products in the Philippines

Djoko, Kisworo (2008) Phenotypic and Genotypic Characterization of Probiotic Bacteria Isolated from Probiotic Dairy Products in the Philippines. Phenotypic and Genotypic Characterization of Probiotic Bacteria Isolated from Probiotic Dairy Products in the Philippines, 137 (1). pp. 78-83. ISSN ISSN 0031 - 7683

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Abstract

Probiotic products that contain live, beneficial microorganisms that provide health benefits have gained popularity among consumers. Fifteen probiotic bacteria were isolated from probiotic dairy products: Chamyto ® and Yakult ® . All the 14 isolates were Gram positive, catalase negative, rods. One isolate was Gram positive, catalase negative, coccus. The biochemical reaction profiles of 6 selected isolates showed that they were all Lactobacillus paracasei. Their ITS-PCR products showed 65% similarity with Lactobacillus paracasei subsp. paracasei. The 16S rRNA gene sequencing results indicated that the identities of the 3 isolates, 1C10, 2Y9, and 3C10 showed complete (100%) similarity with the 16S rRNA gene of Lb. paracasei. However, 1 isolate, 1Y9, showed 98% similarity with the 16S rRNA gene of Enterococcus faecalis.

Item Type: Article
Keywords (Kata Kunci): probiotic, lactic acid bacteria, Lactobacillus paracasei, Enterococcus faecalis
Subjects: S Agriculture > SF Animal culture
Divisions: Fakultas Peternakan
Depositing User: Ir Djoko Kisworo, M.Sc., Ph.D
Date Deposited: 13 Oct 2021 00:43
Last Modified: 13 Oct 2021 00:43
URI: http://eprints.unram.ac.id/id/eprint/24924

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